Supporting Information for Phase Transition Behaviours of the Supported DPPC Bilayer Investigated by Sum Frequency Generation (SFG) and Atomic Force Microscopy (AFM)
نویسندگان
چکیده
منابع مشابه
Investigation of temperature-induced phase transitions in DOPC and DPPC phospholipid bilayers using temperature-controlled scanning force microscopy.
Under physiological conditions, multicomponent biological membranes undergo structural changes which help define how the membrane functions. An understanding of biomembrane structure-function relations can be based on knowledge of the physical and chemical properties of pure phospholipid bilayers. Here, we have investigated phase transitions in dipalmitoylphosphatidylcholine (DPPC) and dioleoyl...
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Temperature-controlled Atomic Force Microscopy (TC-AFM) in Contact Mode is used here to directly image the mechanisms by which melting and crystallization of supported, hydrated DPPC bilayers proceed in the presence and absence of the model peptide WALP23. Melting from the gel L(β)' to the liquid-crystalline L(α) phase starts at pre-existing line-type packing defects (grain boundaries) in absen...
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Supported dipalmitoylphosphatidylcholine (DPPC) bilayers are widely used membrane systems in biophysical and biochemical studies. Previously, short-range positional and orientational order of lipid headgroups of supported DPPC bilayers was observed at room temperature using low deflection noise frequency modulation atomic force microscopy (FM-AFM). While this ordering was supported by X-ray dif...
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In the present study, stability and structure of single and binary mixed monolayers of an unsaturated phospholipid, DOPC, and a saturated phospholipid, DPPC-d75, on the water surface, were explored using the π-A isotherm, atomic force microscopy (AFM) and sum frequency generation (SFG) vibrational spectroscopy in various environments. Our results demonstrated that DOPC in the monolayers becomes...
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